rabbit raised anti goat igg secondary antibody  (Vector Laboratories)

Journal: eLife

Article Title: Ligand-induced shifts in conformational ensembles that describe transcriptional activation

doi: 10.7554/eLife.80140

Figure Lengend Snippet: ( A ) The constitutive activity of M75L receptor was independent of the cell lines used. Fold change of AncSR2 and variants over empty vector in the absence of ligand (cell line: CHO). The error bars associated with each column represent SEM from three independent replicates. Two-tailed unpaired t-test, (***) p<0.0002, (*) p<0.05. ( B ) Western blot experiment showing that the expression of mutant proteins are comparable to WT AncSR2. Figure 2—figure supplement 2—source data 1. Western blot experiments to show that expression of mutant proteins are comparable to WT AncSR2. An equal amount (40 μg) of total protein samples were electrophoresed on SDS-PAGE and transferred to a PVDF membrane. ( A ) Gal4-DBD fused protein was detected using ECI (Thermofisher scientific, USA) after incubation of mouse monoclonal anti-GAL4DBD antibody (sc510, Santacruz Biotechnology, Santa cruz, USA) and horse reddish peroxidase linked secondary antibody (sc-525409, Santacruz Biotechnology, Santa cruz, USA). Negative control was non-transfected Hela cells. ( B ) The same blot stripped off and then restained with rabbit raised anti-actin polyclonal antibody (A2066, Millipore Sigma, USA) and detected by Goat raised horse reddish peroxidase linked secondary antibody (ab97051, Abcam, USA).

Article Snippet: The same blot stripped off and then restained with rabbit raised anti-actin polyclonal antibody (A2066, Millipore Sigma, USA) and detected by Goat raised anti-rabbit horse reddish peroxidase linked secondary antibody (ab97051, Abcam, USA).

Techniques: Activity Assay, Plasmid Preparation, Two Tailed Test, Western Blot, Expressing, Mutagenesis, SDS Page, Membrane, Incubation, Negative Control, Transfection